Table 1. Developmental competence, quality and cryosurvival rate of cloned embryos produced using different types of donor cells.
Animal number | Origin of donor cells | Embryos reconstructed (n) | Fused n (%) | Cleaved n (%) | Blastocysts n (%) | Total cell number | Apoptotic index | Blastocysts vitrified (n) | Blastocysts re-expanded after warming n (%) |
Mu-5926 | Skin | 183 | 183 (100%)a | 181 (99.0±0.65)a | 86 (46.9±3.9)a | 281.7±29.2 | 5.1±0.9a | 27 | 13 (47.5±5.8) |
Fresh semen | 183 | 183 (100%)a | 180 (98.6±1.3)a | 88 (48.8±4.4)a | 299.3±30.3 | 5.3±0.8a | 33 | 21 (63.8±2.8) | |
Frozen-thawed semen | 168 | 168 (100%)a | 159 (94.4±2.0)b | 84 (48.7± 4.5)a | 278.7±20.3 | 5.4±0.7a | 34 | 21 (62.1±2.7) | |
Mu- 4393 | Frozen-thawed semen | 256 | 256 (100%)a | 237 (92.3±3.0)b | 133 (51.4±3.3)a | 272.5±68.4 | 5.7±0.6a | 50 | 29 (55.7±4.9) |
Mu-3567 | Frozen-thawed semen | 245 | 213 (87.0±0.9)b | 182 (92.5±1.0)b | 68 (34.3±3.0)b | 223.0±38.2 | 20.1±4.2b | 20 | 10 (51.5±15.1) |
Data from 12 trials, Values with different letters within the same column differ significantly (P<0.05).