Table 4. Plasmid copy number (PCN) in lactobacilli harboring vectors with the NucA reporter gene.
Strain | Vector | PCN* |
L. plantarum | pLp3050NucA | 2.0±0.2 |
L. plantarum | pLp3050NucA-SH71 | 9.8±2.8 |
L. curvatus | pLp3050NucA-SH71 | 14.9±1.3 |
L. brevis | pLp3050NucA-SH71 | 3.9±1.5 |
L. gasseri | pLp3050NucA-SH71 | 2.7±0.9 |
L. rhamnosus | pLp0373NucA-SH71 | 1.3±0.3 |
*All plasmid copy numbers were calculated from minimum two biological replicates, each analyzed by triplicate qPCR runs. The data shown are the means ± standard deviations.