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. 2014 Mar 10;9(3):e90930. doi: 10.1371/journal.pone.0090930

Figure 3. Determination of chemical nature of epitope for BURK37.

Figure 3

Western blot analysis was performed for BURK37 using crude outer membrane proteins extract (lane 1) and the same antigen priorly subjected to complete protein digestion using 0.02 U of proteinase K for 30 min at 55°C (lane 2). The undistinguishable immunoblot pattern in both the lanes proves that the epitope for BURK37 is a non-proteinaceous compound. The experiment was repeated thrice for reproducibility and the typical LPS ladder pattern was obtained each time.