Table 2. Apparent kinetic constants of recombinant A. meleagris PDH and variant H103Y for selected electron donors, determined with 0.2 mM ferricenium as electron acceptor at 30°C.
AmPDHa | H103Y | |||||
K m | k cat | k cat/K m | K m | k cat | k cat/K m | |
[mM] | [s−1] | [mM−1 s−1] | [mM] | [s−1] | [mM−1 s−1] | |
D-glucose | 0.69±0.09 | 37.8±1.1 | 54.8 | 3.85±0.35 | 27.4±0.5 | 7.1 |
D-galactose | 1.07±0.13 | 47.3±3.0 | 44.2 | 5.29±0.39 | 28.9±2.1 | 5.5 |
Lactose | 128±11.9 | 41.0±8.1 | 0.32 | 395.90±16.27 | 24.7±0.4 | 0.1 |
Data from [36].