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. 2014 Mar 10;9(3):e91145. doi: 10.1371/journal.pone.0091145

Table 2. Apparent kinetic constants of recombinant A. meleagris PDH and variant H103Y for selected electron donors, determined with 0.2 mM ferricenium as electron acceptor at 30°C.

AmPDHa H103Y
K m k cat k cat/K m K m k cat k cat/K m
[mM] [s−1] [mM−1 s−1] [mM] [s−1] [mM−1 s−1]
D-glucose 0.69±0.09 37.8±1.1 54.8 3.85±0.35 27.4±0.5 7.1
D-galactose 1.07±0.13 47.3±3.0 44.2 5.29±0.39 28.9±2.1 5.5
Lactose 128±11.9 41.0±8.1 0.32 395.90±16.27 24.7±0.4 0.1
a

Data from [36].