Table 3. Apparent kinetic constants for selected electron acceptors and oxygen reactivity of recombinant A. meleagris PDH and variant H103Y, determined with 25 mM D-glucose as electron donor at 30°C.

	AmPDH			H103Y
	K m	k cat	k cat/K m	K m	k cat	k cat/K m
	[mM]	[s−1]	[mM−1 s−1]	[mM]	[s−1]	[mM−1 s−1]
Fc+PF6 (pH 8.5)	0.16±0.04a	130±11a	812.5	0.10±0.01	26.0±0.9	260.0
1,4-BQ (pH 4)	1.38±0.28a	65.4±5.5a	47.4	3.64±0.47	13.6±1.1	4.0
DCIP (pH 4)	0.14±0.01	40.7±4.1	290.7	0.02±0.01	2.8±0.5	140.0
O2 [µM min−1 mg−1]	0.095±0.003			0.500±0.033

^aData from [36].