Skip to main content
. 2014 Mar 10;9(3):e91175. doi: 10.1371/journal.pone.0091175

Figure 1. Comparison of the effects of purines on Na+ dependent Na,K-ATPase activity in membranes from rat and human skeletal muscle.

Figure 1

The assay was performed at 0.5(control, dark column). a. Rat muscle. The bars represent the Na+ stimulated Na,K-ATPase activity (+SEM) at 40 mM Na+ (calculated from the difference between the activity at 0 and 40 mM Na+) after pre-incubation with purines (ADP, MeS-ADP, UTP, and UDP) in the presence of 0.5 mM ATP. Mean+SE shown. *indicates different from the activity with 0.5 mM ATP. #indicates different from UTP, n = 8. b. Human muscle. The effect of ADP, MeS-ATP, UTP, ATPγS, and UDP) in the presence of 0.5 mM ATP and 40 mM Na+ (control). In one series of experiments the P2Y antagonist suramin (100 µM) was added together with UTP, n = 6.