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. 2013 Dec 20;196(3):605–613. doi: 10.1534/genetics.113.160846

Figure 2.

Figure 2

Each 2A viral peptide allows the efficient production of two independent polypeptides at different developmental stages. (A) Quantification at different developmental stages of the expected localization patterns (GFP in the whole cell, HISTONE::mCherry in the nucleus) observed in lines expressing myo-3p::GFP::E2A::histone::mCherry, myo-3p::GFP::P2A*::histone::mCherry, or myo-3p::GFP::SL2::histone::mCherry. (B) Patterns of localization of GFP and HISTONE::mCherry in muscle cells of myo-3p::GFP::F2A::histone::mCherry or myo-3p::GFP::P2A*::histone::mCherry transgenic worms at the indicated developmental stages. n, nucleus; c, cytosol. See Table S3 for information on the different transgenic lines obtained.