Table 3. Primers used in this study.

	Primer name	Primer sequence	Imbedded RE
1	GAD·Fob1·5′	5′-ATGCTA GGATCCAC4ACGAAACCGCGTTACAATG22	BamHI
2	GAD·Fob1·3′	5′-ATGCTA GTCGAC1701TTACAATTCCATTGATGTG1683	SalI
3	Fob1·Dom2·3′	5′-ATGCTA AGATCT A 1258CATTAGCAAGGGCAAAAG1241	BglII
4	Fob1·Dom3·5′	5′-ATGCTA GGATCC1259AAGCGGATAATAGCTGTAAC1278	BamHI
5	Fob1·N-term·3′	5′-ACGT GTCGAC849AATTGGAACCCTAGCAAATG829	SalI
6	Fob1·C-term·5′	5′-ACGT GGATCC850ACTTCGTAACATCAAGCATCTTA G868	BamHI
7	E420V 3′	5′-ACGTACGTG1291GAATTC CATTATTGTTACAGCTATTATCCGCAACgTTAGCAAGGG1247	EcoRI/AclI

The underlined sequences reflect restriction sites imbedded for cloning purposes. The nucleotide in bold in primer 7 was mutated to create the E420V mutation, which also created the new AclI restriction site. The superscript numbers within the primer sequences define where in the FOB1 DNA sequence the primers reside.

RE, restriction enzyme.