Figure 3.

Mutants affected in hyphal fusion show increased levels of NBT reduction. (A) Quantitative measurement (fold change) of NBT precipitates after 30 min of incubation in at least three replicates. Gray bars indicate hyphal fusion-deficient strains. Hyphal fusion defect correlates with sterility, except for sterile mutant ∆gsa3∆ste12, which can undergo normal hyphal fusion. Normalization was done in reference to wild type. (B) Detailed view of sexual (black arrowheads) and vegetative (white arrowheads) structures stained by NBT. Bar, 50 µm, 1 = ascospores.