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. 2014 Mar 10;9(3):e91359. doi: 10.1371/journal.pone.0091359

Figure 7. Amino acids 11–149 in RTA are required for degradation of vFLIP.

Figure 7

A. Illustration depicting the RTA truncation mutants used in this study. Note that the rabbit RTA antibody recognizes amino acids 527–539, so not all mutants were detected by western blot. B. 293T cells were transfected with vFLIP and wild type or mutant RTA where indicated. 72 h post-transfection cells were harvested and analyzed by SDS-PAGE followed by western blot against myc(vFLIP), RTA and B-actin. C. 293T cells were transfected with V5 tagged RTA 11–149 and vFLIP where indicated. 72 h post-transfection cells were harvested and analyzed by SDS-PAGE followed by western blot against myc(vFLIP), RTA and B-actin.