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. 2014 Mar 10;9(3):e91370. doi: 10.1371/journal.pone.0091370

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© 2014 Korrer, Routes

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A) Representative gating strategy to determine immune infiltrate of MCA-205-OVA tumors. B6 mice were administered 1×10⁵ MCA-205-OVA cells, and tumors were excised and digested when the tumor reached 15 mm. The immune cells which infiltrated the tumor were determined by flow cytometry. CD45⁺, live cells were first gated. Macrophages (CD45⁺CD11b⁺ CD11c^lo F4/80⁺GR-1⁻), myeloid-derived suppressor cells [(MDSC) CD45⁺CD11b⁺ CD11c^lo GR-1⁺], CD4 T cells (CD45⁺CD11b⁻CD3⁺CD4⁺), CD8 T cells (CD45⁺CD11b⁻CD3⁺CD8⁺), and NK cells (CD45⁺CD11b⁻CD3⁻CD8⁻CD4⁻NK1.1⁺) were quantified in B as the percentage of CD45⁺ cells. Tregs were <1%, not shown in figure. Data shown is the mean ± SEM from three mice.