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. 2014 Mar 10;9(3):e91307. doi: 10.1371/journal.pone.0091307

Figure 5. MiR-214 has no effect on cell apoptosis in four GC cells.

Figure 5

(A, C, E, G) Cells were labeled with Annexin V-PE/7-AAD or Annexin V-FITC/PI, and analyzed by flow cytometry. All these figures are representative of three independent assays. Quadrant statistics: necrosis or mechanically-injured cells in upper left (UL), late apoptosis cells in upper right (UR), viable cells in lower left (LL) and early apoptosis cells in lower right (LR). (B, D, F, H) The percentage of early apoptosis cells, late apoptosis cells and total apoptosis cells were respectively compared between LV3hsa-miR-214 transfected group and NC group, or between miR-214 inhibitor-transfected group and inhibitor NC group. Our data demonstrated that LV3-hsa-miR-214 or miR-214 inhibitor has no effect on cell apoptosis in SGC7901, MKN45, MKN28 and BGC823 cells (P>0.05). Although we observed a trend of pro-apoptosis ability of miR-214 in MKN45 cell line, however, the difference was not statistically significant (P = 0.0950).