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. 2014 Mar 10;9(3):e91307. doi: 10.1371/journal.pone.0091307

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© 2014 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The complementary sequences of the CSF1 mRNA 3′-UTR are shown with the miR-214 sequence. (B) Luciferase activity in MKN45 and BGC823 cells transfected with miR-214 and pmirGLO-CSF1 was significantly deceased compared with the negative controls (P = 0.0227 and 0.0093, respectively). (C) After miR-214 inhibitor was transfected, luciferase activity was dramatically increased in MKN45 and BGC823 cells compared with the controls (P = 0.0115 and 0.0085, respectively). (D) The effect of miR-214 on CSF1 levels were tested in GC cell lysates by western blot. (E). Our data showed that the relative CSF1 expression was significantly decreased in cells transfected with miR-214 precrusor as compared to the cells transfected with negative control (P = 0.0037 and 0.0066, respectively).