Figure 3. Frequency and function of regulatory T cells from p18^ink4c−/− mice.

Spleens from naïve, 4-week-old WT (left panel) and p18^ink4c−/− (right panel) mice were stained for CD4 and CD25 to identify Treg (A). CFSE-labeled CD4+CD25− conventional T cells purified from wild-type mice were used as targets for CD4+CD25+ Treg purified from either WT (dark gray bars) or p18^ink4c−/− (light gray bars) mice in a standard anti-CD3-stimulated suppression assay (B). CFSE-labeled CD4+CD25− conventional T cells purified from either WT (dark gray bars) or p18^ink4c−/− (light gray bars) mice were used as targets for wild-type CD4+CD25+ Treg (C). Data are plotted as the mean +/−SD of triplicate cultures.