Figure 5. Effect of p18^ink4c deficiency on the development of graft-vs.-host disease.

C57BL/6 X BALB/c F1 mice aged 4–6 weeks were subjected to irradiation (600 cGy) 6 hours before adoptive transfer of 10×10⁶, 5×10⁶ or 1×10⁶ B6 WT (dark gray symbols, n = 5) or p18^ink4c−/− (light gray symbols, n = 5) donor splenocytes. Recipient mice were weighed daily and observed for outward signs of GVHD. On day 13, recipient mice were weighed (A) and sacrificed to determine the frequency of host CD19+ B lymphocytes in the spleen by flow cytometry (B).