Figure 1. CD4 T cells are the most frequent IL-10 expressing cell type in chronic LCMV infection.

Adult IL10-GFP reporter mice were infected with Cl13 and spleens harvested at the indicated days post-infection. (A) The frequency of IL-10 positive cells, as measured by IL-10 GFP reporter activity, in total splenocytes is shown. (B) Splenocytes from IL-10 GFP reporter mice were stained for CD11c, CD90, B220, CD4 and CD8 at the indicated times post-infection. Shown is the frequency of IL10^pos splenocytes (as determined by GFP reporter expression) that stain positively for CD4 T cell, CD8 T cell, B cell and DC markers. (C) IL-10 GFP reporter mice (top three rows) or wildtype C57BL/6 non-reporter mice (bottom two rows) were infected (as indicated) and spleens harvested 15 days post infection. Dot plots depict unstimulated IL-10 GFP reporter expression directly ex vivo. Naïve IL-10 GFP reporter mice indicate baseline IL-10 expression. Naïve and Cl13 infected wildtype C57BL/6 are used as autofluorescence gating controls. (A and B) n = 6 for Cl13 infected mice and n = 5 for uninfected. (C) n = 4 for Cl13, 3 for Arm, 2 naïve and 2 Cl13 B6 nonreporter gating controls. Data are representative of one of three (A) or four (B) independent experiments. Standard Deviation is shown.