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. 2014 Mar 10;9(3):e90855. doi: 10.1371/journal.pone.0090855

Figure 5. Cl13 exposed DCs induce IL-10 expression in naïve polyclonal CD4T cells.

Figure 5

Adult C57BL/6 females were infected with Cl13 or Arm and naïve spleens harvested 7 days post-infection. Splenic DCs were sorted based on infected state (NPpos infected, NPneg exposed) and CD8α expression (CD8αneg used exclusively). (A) 1×104 sorted DCs were cultured with polyclonal CD4T cells from wildtype or IL-10 knock out C57BL/6 mice. Standard deviation is shown. (D) Polyclonal CD4T cells were harvested from OTII transgenic mice and transferred into either naïve B6.SJL or B6.SJL infected with Cl13 four days prior to T cell transfer. Spleens were harvested 3 days post-transfer (7 days post infection), stimulated in vitro with OVA peptide and analyzed by ICCS for IL-10 expression. Shown is data from 2 experiments with n = 3 Cl13 and n = 4 for naïve. Standard error of the mean is shown.