Fig. 4.

E₂ increases GHSR mRNA expression in Kiss1 neurons of the ARC. A and B: fluorescent images showing expression of GHSR mRNA (hybridization signal, silver grains) in Kiss1 neurons [green fluorescent protein (GFP) immunoreactivity] of the ARC of OVX (A) and OVX + E₂ mice (B). Arrows indicate dual-labeled neurons. Note the higher expression of GHSR mRNA (silver grains) and higher no. of dual-labeled neurons (GHSR + Kiss1) in the ARC of OVX + E₂ mice. Scale bar, 100 μm.