Table 2.

Changes in hepatic mRNA expression of key genes involved in lipid metabolism

Gene Symbol	+/Dsk5 Relative Expression	+/+ Relative Expression	Fold Change	P Value
HMG-CoA Reductase	52.08	18.87	2.76	0.019
SREBP2	39.22	17.28	2.27	0.042
SREBP1c	22.73	7.83	2.90	0.036
LDLR	13.86	5.85	2.37	0.097
FAS	3.44	1.00	3.44	0.020

The relative amounts of mRNA for 3-hydroxy-3-methyl-glutaryl (HMG)-CoA reductase, sterol-regulatory element-binding protein (SREBP)2, SREBP1c, low-density lipoprotein receptor (LDLR), and fatty acid synthase (FAS) were measured by quantitative RT-PCR assay. The amount was calculated relative to the mRNA for GAPDH. The analysis is derived from 3-mo-old +/+ (n = 4) and +/Dsk5 (n = 5) male mice.