Fig. 5.

Inhibition of Notch1 signaling pathway reduced surviving Dclk1-positive cells after radiation injury. C57Bl/6 mice in control group (A) or difluorophenacetyl-l-alanyl-S-phenylglycine t-butyl ester (DAPT)-treated group (B) are subjected to TBI. Post-IR (24 h), the small intestines were isolated, fixed, processed, and stained with anti-Dclk1 antibody. Dclk1-positive cells in one cross section were indicated by arrows in A and B. The magnification is ×40 for the cross section pictures, and approximately ×600 for the inset. The average number of Dclk1 positive cells per cross section was counted for total of 80 cross sections and presented in C. Values in the bar graph are given as averages ± SE. *P < 0.01, statistically significant difference compared with control.