Table 3.
Real-time RT-PCR analysis of intestinal gene expression
| 5 mo |
12 mo |
|||||
|---|---|---|---|---|---|---|
| Gene | Wild-type | Ostα−/− | Ostα−/−/wild-type | Wild-type | Ostα−/− | Ostα−/−/wild-type |
| Intestinal mediators of bile acid synthesis, transport, and signaling | ||||||
| Proximal small intestine | ||||||
| Asbt | BD | BD | BD | BD | BD | BD |
| Fgf15 | BD | BD | BD | BD | BD | BD |
| Fxr | 0.31 ± 0.03 | 0.33 ± 0.04 | 1.05 ± 0.12 | 0.25 ± 0.02 | 0.33 ± 0.02* | 1.29 ± 0.08* |
| Ibabp | BD | BD | BD | BD | BD | BD |
| Mrp3 | 0.84 ± 0.2 | 0.84 ± 0.04 | 0.99 ± 0.05 | 1.3 ± 0.2 | 0.72 ± 0.08* | 0.57 ± 0.07* |
| Shp | 0.021 ± 0.002 | 0.020 ± 0.003 | 0.93 ± 0.16 | 0.010 ± 0.002# | 0.029 ± 0.002*# | 2.78 ± 0.18* |
| Middle small intestine | ||||||
| Asbt | 0.12 ± 0.02 | 0.25 ± 0.02* | 2.11 ± 0.18* | 0.11 ± 0.02 | 0.12 ± 0.04# | 1.14 ± 0.40 |
| Fgf15 | 0.22 ± 0.05 | 0.091 ± 0.02* | 0.41 ± 0.07* | 0.12 ± 0.03 | 0.098 ± 0.02 | 0.85 ± 0.15 |
| Fxr | 0.34 ± 0.03 | 0.36 ± 0.02 | 1.07 ± 0.06 | 0.35 ± 0.02 | 0.48 ± 0.05* | 1.38 ± 0.14* |
| Ibabp | 0.42 ± 0.10 | 26 ± 5* | 61.7 ± 13.1* | 0.25 ± 0.05 | 29 ± 3* | 116 ± 13.8* |
| Mrp3 | 0.32 ± 0.05 | 0.51 ± 0.03 | 1.58 ± 0.12* | 0.51 ± 0.03# | 0.34 ± 0.06*# | 0.66 ± 0.12* |
| Shp | 0.0072 ± 0.002 | 0.022 ± 0.002* | 3.13 ± 0.25* | 0.0096 ± 0.001 | 0.020 ± 0.002* | 2.06 ± 0.20* |
| Distal small intestine | ||||||
| Asbt | 0.82 ± 0.04 | 0.82 ± 0.05 | 1.00 ± 0.06 | 0.64 ± 0.07 | 0.94 ± 0.07* | 1.46 ± 0.11* |
| Fgf15 | 3.88 ± 0.4 | 6.45 ± 1.3 | 1.66 ± 0.34 | 5.02 ± 0.37 | 6.96 ± 1.7 | 1.39 ± 0.33 |
| Fxr | 0.55 ± 0.04 | 0.68 ± 0.05 | 1.22 ± 0.09 | 0.53 ± 0.03 | 0.71 ± 0.09 | 1.34 ± 0.18 |
| Ibabp | 41 ± 2 | 91 ± 6* | 2.24 ± 0.14* | 42 ± 3 | 100 ± 9* | 2.37 ± 0.22* |
| Mrp3 | 0.42 ± 0.04 | 0.91 ± 0.07* | 2.15 ± 0.16* | 0.40 ± 0.04 | 0.65 ± 0.1* | 1.63 ± 0.28* |
| Shp | 0.017 ± 0.001 | 0.067 ± 0.02* | 3.94 ± 1.04* | 0.015 ± 0.002 | 0.074 ± 0.02 | 4.89 ± 1.51 |
| Intestinal lipid transporters | ||||||
| Proximal small intestine | ||||||
| Abca1 | 0.73 ± 0.09 | 0.28 ± 0.08* | 0.39 ± 0.10* | 0.66 ± 0.03 | 0.21 ± 0.04* | 0.32 ± 0.06* |
| Abcg5 | 1.3 ± 0.1 | 1.1 ± 0.1 | 0.84 ± 0.09 | 1.7 ± 0.1# | 1.6 ± 0.1# | 0.95 ± 0.08 |
| Abcg8 | 0.84 ± 0.1 | 0.85 ± 0.07 | 1.01 ± 0.08 | 1.1 ± 0.1 | 1.2 ± 0.1 | 1.02 ± 0.10 |
| Fat | 0.92 ± 0.2 | 1.2 ± 0.1 | 1.27 ± 0.16 | 0.36 ± 0.07 | 0.65 ± 0.1# | 1.78 ± 0.27 |
| Npc1L1 | 0.94 ± 0.1 | 0.81 ± 0.2 | 0.86 ± 0.17 | 1.2 ± 0.2 | 1.8 ± 0.2# | 1.54 ± 0.19 |
| Srb1 | 0.084 ± 0.005 | 0.091 ± 0.005 | 1.09 ± 0.06 | 0.16 ± 0.01# | 0.27 ± 0.01*# | 1.72 ± 0.08* |
| Middle small intestine | ||||||
| Abca1 | 0.68 ± 0.1 | 0.16 ± 0.01* | 0.24 ± 0.01* | 0.79 ± 0.05 | 0.20 ± 0.02* | 0.25 ± 0.03* |
| Abcg5 | 1.3 ± 0.1 | 0.86 ± 0.04 | 0.66 ± 0.03* | 1.1 ± 0.09 | 0.94 ± 0.09 | 0.82 ± 0.08 |
| Abcg8 | 0.87 ± 0.1 | 0.65 ± 0.03 | 0.76 ± 0.04* | 0.64 ± 0.06 | 0.71 ± 0.04 | 1.10 ± 0.07 |
| Fat | 0.017 ± 0.001 | 0.019 ± 0.002 | 1.12 ± 0.10 | 0.024 ± 0.002# | 0.027 ± 0.002# | 1.11 ± 0.08 |
| Npc1L1 | 1.2 ± 0.2 | 1.3 ± 0.1 | 1.07 ± 0.08 | 1.4 ± 0.2 | 1.3 ± 0.2 | 0.98 ± 0.16 |
| Srb1 | 0.013 ± 0.001 | 0.010 ± 0.0006 | 0.78 ± 0.05 | 0.016 ± 0.001 | 0.018 ± 0.002# | 1.16 ± 0.11 |
| Distal small intestine | ||||||
| Abca1 | 0.42 ± 0.08 | 0.28 ± 0.03* | 0.68 ± 0.06* | 0.62 ± 0.02 | 0.20 ± 0.04* | 0.32 ± 0.07* |
| Abcg5 | 1.14 ± 0.07 | 0.76 ± 0.08* | 0.66 ± 0.07* | 0.96 ± 0.03 | 0.67 ± 0.08 | 0.70 ± 0.08 |
| Abcg8 | 1.1 ± 0.1 | 0.73 ± 0.05* | 0.69 ± 0.05* | 0.84 ± 0.01 | 0.75 ± 0.1 | 0.88 ± 0.13 |
| Fat | 0.66 ± 0.05 | 0.76 ± 0.07 | 1.16 ± 0.10 | 0.61 ± 0.06 | 0.83 ± 0.07 | 1.37 ± 0.11* |
| Npc1L1 | 2.0 ± 0.2 | 0.91 ± 0.09* | 0.46 ± 0.05* | 1.6 ± 0.1 | 0.96 ± 0.08* | 0.61 ± 0.05* |
| Srb1 | 0.019 ± 0.001 | 0.018 ± 0.001 | 0.95 ± 0.06 | 0.020 ± 0.0007 | 0.018 ± 0.002 | 0.92 ± 0.10 |
Values are means ± SE (n = 6). Small intestine from 5- and 12-mo-old mice was sectioned into 3 equal pieces, with proximal section attached to the stomach and distal section attached to the cecum. Columns labeled wild-type and Ostα−/− show gene expression normalized to β-actin expression; columns labeled Ostα−/−/wild-type show ratio of Ostα−/− to wild-type expression.
BD, below detection.
*
P < 0.05 vs. wild-type.
#
P < 0.05 vs. 5 months.