Table 4.
Real-time RT-PCR analysis of hepatic gene expression
| 5 Months |
12 Months |
|||||
|---|---|---|---|---|---|---|
| Gene | Wild-type | Ostα−/− | Ostα−/−/wild-type | Wild-type | Ostα−/− | Ostα−/−/wild-type |
| Hepatic mediators of bile acid synthesis, transport, and signaling | ||||||
| Bsep | 0.28 ± 0.01 | 0.21 ± 0.02 | 0.73 ± 0.09 | 0.20 ± 0.01# | 0.19 ± 0.02 | 0.94 ± 0.08 |
| Cyp27a1 | 1.7 ± 0.1 | 1.63 ± 0.07 | 0.96 ± 0.04 | 1.1 ± 0.1# | 0.85 ± 0.04# | 0.79 ± 0.03 |
| Cyp7a1 | 0.14 ± 0.01 | 0.035 ± 0.01* | 0.26 ± 0.08* | 0.085 ± 0.01# | 0.044 ± 0.01* | 0.52 ± 0.13* |
| Cyp8b1 | 0.090 ± 0.01 | 0.071 ± 0.007 | 0.78 ± 0.08 | 0.039 ± 0.002# | 0.032 ± 0.004# | 0.82 ± 0.12 |
| Fxr | 0.036 ± 0.003 | 0.046 ± 0.01 | 1.28 ± 0.28 | 0.025 ± 0.002# | 0.027 ± 0.003 | 1.10 ± 0.12 |
| Shp | 0.029 ± 0.04 | 0.022 ± 0.04 | 0.77 ± 0.15 | 0.017 ± 0.001# | 0.013 ± 0.001 | 0.79 ± 0.09 |
| Hepatic mediators of lipid synthesis | ||||||
| Acaca | 0.035 ± 0.001 | 0.036 ± 0.003 | 1.05 ± 0.07 | 0.016 ± 0.001# | 0.022 ± 0.001# | 1.40 ± 0.08 |
| Acacb | 0.023 ± 0.003 | 0.025 ± 0.002 | 1.12 ± 0.07 | 0.0095 ± 0.0006# | 0.016 ± 0.002*# | 1.68 ± 0.16* |
| Dgat2 | 0.65 ± 0.08 | 0.54 ± 0.03 | 0.83 ± 0.05 | 0.60 ± 0.08 | 0.60 ± 0.06 | 0.99 ± 0.10 |
| Fas | 0.039 ± 0.004 | 0.053 ± 0.004 | 1.37 ± 0.11 | 0.018 ± 0.002# | 0.045 ± 0.004* | 2.44 ± 0.23* |
| Fdft1 | 0.11 ± 0.02 | 0.28 ± 0.02* | 2.47 ± 0.19* | 0.033 ± 0.004# | 0.13 ± 0.01*# | 3.90 ± 0.34* |
| Gpat | 0.090 ± 0.008 | 0.086 ± 0.003 | 0.96 ± 0.03 | 0.075 ± 0.003 | 0.090 ± 0.009 | 1.20 ± 0.12 |
| Hmgcr | 0.084 ± 0.008 | 0.21 ± 0.03* | 2.45 ± 0.36* | 0.046 ± 0.006# | 0.11 ± 0.01*# | 2.47 ± 0.32* |
| Insig1 | 0.28 ± 0.02 | 0.50 ± 0.01* | 1.78 ± 0.05* | 0.10 ± 0.01# | 0.16 ± 0.01*# | 1.66 ± 0.14* |
| Insig2 | 0.14 ± 0.02 | 0.18 ± 0.04 | 1.22 ± 0.30 | 0.12 ± 0.01 | 0.12 ± 0.02 | 1.04 ± 0.14 |
| Lpin1 | 0.019 ± 0.002 | 0.064 ± 0.01* | 3.37 ± 0.73* | 0.013 ± 0.002 | 0.018 ± 0.002# | 1.45 ± 0.15 |
| Lpin2 | 0.21 ± 0.03 | 0.30 ± 0.03 | 1.43 ± 0.16 | 0.13 ± 0.01# | 0.13 ± 0.01# | 0.97 ± 0.08 |
| Lxrα | 0.061 ± 0.005 | 0.052 ± 0.003 | 0.85 ± 0.05* | 0.042 ± 0.006 | 0.042 ± 0.003 | 1.00 ± 0.07 |
| Mvd | 0.0053 ± 0.002 | 0.015 ± 0.002* | 2.83 ± 0.30* | 0.0019 ± 0.0003 | 0.0091 ± 0.001*# | 4.88 ± 0.62* |
| Pparγ | 0.20 ± 0.04 | 0.21 ± 0.05 | 1.03 ± 0.25 | 0.15 ± 0.01 | 0.12 ± 0.01 | 0.79 ± 0.08 |
| Scap | 0.072 ± 0.004 | 0.076 ± 0.003 | 1.05 ± 0.05 | 0.043 ± 0.001# | 0.058 ± 0.007 | 1.32 ± 0.15 |
| Srebp1a | 0.0127 ± 0.0007 | 0.011 ± 0.001 | 0.84 ± 0.07 | 0.0074 ± 0.001# | 0.0068 ± 0.0002# | 0.93 ± 0.03 |
| Srebp1c | 0.061 ± 0.01 | 0.034 ± 0.003* | 0.55 ± 0.05* | 0.037 ± 0.005 | 0.023 ± 0.003*# | 0.63 ± 0.07* |
| Srebp2 | 0.043 ± 0.002 | 0.055 ± 0.007 | 1.28 ± 0.16 | 0.029 ± 0.003# | 0.042 ± 0.003* | 1.43 ± 0.12* |
| Hepatic mediators of cholesterol transport | ||||||
| Abca1 | 0.22 ± 0.02 | 0.17 ± 0.01 | 0.76 ± 0.07 | 0.10 ± 0.02# | 0.089 ± 0.005# | 0.90 ± 0.05 |
| Abcg5 | 0.053 ± 0.005 | 0.063 ± 0.006 | 1.19 ± 0.11 | 0.034 ± 0.005# | 0.039 ± 0.003# | 1.14 ± 0.09 |
| Abcg8 | 0.070 ± 0.007 | 0.085 ± 0.008 | 1.20 ± 0.11 | 0.042 ± 0.007# | 0.048 ± 0.002# | 1.13 ± 0.05 |
| Ldlr | 0.117 ± 0.007 | 0.14 ± 0.02 | 1.20 ± 0.15 | 0.061 ± 0.007# | 0.083 ± 0.002# | 1.37 ± 0.04 |
Values are means ± SE (n = 6). Liver from 5- and 12-mo-old mice was used to analyze gene expression. Columns labeled wild-type and Ostα−/− show gene expression normalized to β-actin expression; columns labeled Ostα−/−/wild-type show ratio of Ostα−/− to wild-type expression.
BD, below detection.
*
P < 0.05 vs. wild-type.
#
P < 0.05 vs. 5 months.