Fig. 1.

ANG II stimulates the 50-pS K channel. A channel recording showing the effect of ANG II (50 nM) on the 50-pS K channel activity in the basolateral membrane of the thick ascending limb (TAL). The experiment was performed in a cell-attached patch with 140 mM KCl in the pipette and 140 mM NaCl/5 mM KCl in the bath. Top trace shows the time course of the experiment. Three parts of the trace, indicated by numbers, are extended to show the fast time resolution. The holding potential was 0 mV, and the channel closed current is indicated by “C.”