Fig. 7.

Superoxide anions are required for the effect of ANG II on the K channel. A: channel recording showing the effect of 50 nM ANG II on the 50-pS K channels in the TAL treated with 10 μM sodium 4,5-dihydroxybenzene-1,3-disulfonate (tiron). The experiments were performed in a cell-attached patch and the holding potential was 0 mV. Top trace is a time course of the experiment. Two parts of the trace, indicated by numbers, are extended to show the fast time resolution. B: bar graph summarizes the results from 6 experiments.