Fig. 4.
Deletion of exon 11-encoded region of the α-subunit increases channel open probability (Po). Patch-clamp recordings were performed with oocytes expressing either αβγ (A) or αΔExon11βγ (B). Cell-attached patches were clamped at −100 mV (membrane potential). Both bath and pipette solutions contained 110 mM Na+. Downward deflections indicate channel openings. C, closed state; O1 and O2, open states. The scale bar is for both traces. C: averaged Po of WT and mutant channels. Po was estimated using the single channel analysis function of ClampFit 10. D: averaged unitary currents at −100 mV. Unitary currents in each patch were obtained from the event analyses after single channel searching by ClampFit 10.