Fig. 3.

Laminin-332 is aberrantly expressed in human autosomal recessive polycystic kidney disease (ARPKD) kidney epithelia. A: relative expression levels of laminin α₃-, β₃-, and γ₂-chains in human ARPKD cells compared with those of normal human kidney (NHK) cells. There was a significant 2-fold increase in mRNA levels of laminin-γ₂ in ARPKD cells compared with NHK cells. B: frozen kidney sections of a human subject with no kidney disease stained with a monoclonal laminin-332 antibody (red) and Dolichos biflorus agglutinin (DBA; green) demonstrated no red (laminin-332) staining in collecting ducts. C: laminin-332 (red) and DBA (green) staining in cystic structures (arrows) and precystic tubules of a typical kidney section procured from an ARPKD/congestive heart failure patient. D: laminin-332 staining alone of the image in C for clarity.