Fig. 3.
Expression of renin-angiotensin system (RAS) components in preglomerular VSMCs. A: immunostainings of AGT, renin, angiotensin-converting enzyme (ACE), and ANG II receptor (AT1R) in preglomerular VSMCs shown in red. Nuclei were stained by DAPI (middle). Merged images are shown on the right. Magnification: ×600. B: Western blot analyses of AGT, AT1R, and β-actin protein expression in preglomerular VSMCs. C: RT-PCR analyses of AGT, AT1a, AT1b, and β-actin in preglomerular VSMCs (left) and aortic VSMCs (right).