Fig. 5.

Effects of ANG II on AGT expression. A: preglomerular VSMCs (n = 12) and aortic VSMCs (n = 8) were incubated with ANG II for 24 h, and then, qRT-PCR analysis was performed. B: preglomerular VSMCs were incubated with ANG II for 24 h, and then Western blotting using anti-AGT and anti-β-actin antibodies was performed (n = 3). C: preglomerular VSMCs were incubated with olmesartan (10 nmol/l) for 30 min and then ANG II (100 pmol/l) for 24 h (n = 8–16). Thereafter, AGT expression levels were measured by qRT-PCR. Values are means ± SE. IB, immunoblot. *P < 0.05, **P < 0.01 vs. control. ^##P < 0.01 vs. ANG II-treated group.