Fig. 8.

Effects of ROCK inhibition on ANG II-induced NF-κB activation. Preglomerular VSMCs were pretreated with H-1152 (10 μmol/l) for 30 min and then with ANG II (100 pmol/l) for 24 h. A: p65 (an NF-κB subunit) mRNA expression in preglomerular VSMCs was determined by qRT-PCR (n = 8). Values are means ± SE. *P < 0.05 vs. control. B: effects of H-1152 on ANG II-induced translocation of p65 into nuclei. Preglomerular VSMCs were treated with ANG II (100 pmol/l) for 15 min. p65 was stained with anti-p65 antibody (green). a: control. b: ANG II-treated cell. c: ANG II with ROCK inhibitor-treated cell. d: ROCK inhibitor-treated cell. Nuclei were stained with DAPI (blue). Magnification: ×600.