Fig. 7.

Effect of high glucose and high insulin combination (HGHI, glucose concentration 15 mmol/l insulin concentration 50 nmol/l) on cellular content and localization of pRb in mouse C2C12 myoblasts proliferating for 24 h in 2 % FBS/DMEM (Control, Ctrl) or in the presence of experimental factors. Cell cultures were stained with antibodies against pRb (green) and simultaneous nuclear staining with 7-AAD (red) was performed. Images are representative of ten independent fields in three separate experiments. Bar 20 μm. The IOD values of pRb-related green fluorescence were evaluated using MicroImage analysis system (Olympus Optical Poland). The data were presented as mean ± SD with n = 10/treatment conditions of total fluorescence of fields with similar cell density and as a nucleus/cytoplasm immunofluorescence ratio