Fig. 6.

RESV-mediated wound healing in HAEC under LSS is dependent on signaling though estrogen receptor (ER)-α. Representative Western blot (A) and its quantitation (B) showing expression of ER-α 18 h after treatment with 5 μM RESV and/or LSS. C: quantitation of HAEC wound healing under LSS in cells transfected with scrambled or small interfering (si)RNA against ER-α. D: nuclei were identified using DAPI staining and Ki67 was used to determine cell proliferation within the control region. E: representative Western blot showing levels of ER-α, endothelial nitric oxide synthase (eNOS), phospho-ERK (P-ERK), and ERK proteins in HAEC under LSS and transfected with scrambled or siRNA against ER-α. P-ERK levels were quantified as a fold increase in P-ERK/ERK compared with vehicle-treated cells transfected with scrambled siRNA. *P < 0.05, compared with the appropriate vehicle control; n = 3.