Fig. 4.
Role of kinases for UcnII-induced nuclear export of NFATc1. A: confocal images of subcellular NFATc1-GFP distribution in control (a), in the presence of UcnII (b), and UcnII plus kinase inhibitors KT5823 (PKG1 inhibition; c), asterpaullone [ALP; glycogen synthase kinase-3β (GSK3β) inhibition; d), ALP + ODQ (sGC inhibition; e), ALP + SB203580 (p38 kinase inhibition) + SP600125 [c-Jun NH2-terminal kinase (JNK) inhibition; f], and ALP + SB203580 + SP600125 + KT5823 (g). B: average data of RNFATc1 normalized to control (left ordinate) and expressed as %change relative to UcnII (right ordinate). *P < 0.001 vs. UcnII. Scale bar = 20 μm.