Fig. 8.

UcnII-induced NFAT-regulated transcriptional activity in HF myocytes. A: HF cardiac myocytes were infected with IL2-RFP to measure endogenous NFAT-dependent transcriptional activity. Averaged normalized data of IL2-RFP fluorescence in control and after UcnII treatment. B: HF cells were coinfected with Luc-NFAT-GFP and NFATc1-GFP for measurements of NFATc1-regulated transcriptional activity. Averaged normalized relative luciferase activity in control and in the presence of UcnII. C: average normalized NFATc3-dependent relative luciferase activity in HF myocytes in control and after UcnII treatment. *P < 0.05 vs. control. Numbers in parentheses indicate numbers of individual cells treated (A) or numbers of hearts (B and C). In B and C, data for UcnII effects were normalized to control values obtained from the same heart.