Fig. 3.

PostC increased myocardial protein SNO. Top: representative two-dimensional (2D) CyDye-maleimide difference gel electrophoresis (DIGE) gel from three independent experiments was scanned at each of the distinct wavelengths of the fluors, showing a pattern of protein SNO for that particular treatment group. Bottom: overlaid image of Cy3-maleimide (I/R control, green) vs. Cy5-maleimide (PostC, red). Protein spots (1–13) showing a change of at least 25% or higher in PostC hearts compared with I/R-control were picked for MS/MS analysis and are listed in Table 2.