Table 1.

Evaluation of cardiac contractile function in Langendorff-perfused mouse hearts

			Preischemic Equilibration					End of Reperfusion
Heart Samples	n	BW, g	FR, ml/min	HR, beats/min	LVDP, cmH2O	+dP/dt, cmH2O/ms	−dP/dt, cmH2O/ms	FR, ml/min	HR, beats/min	LVDP, cmH2O	+dP/dt, cmH2O/ms	−dP/dt, cmH2O/ms
I/R-Control	9	27.2 ± 0.6	2.6 ± 0.2	346 ± 18	144 ± 8	8.1 ± 0.5	−5.8 ± 0.2	1.7 ± 0.2	338 ± 10	33 ± 6	4.2 ± 0.4	−3.4 ± 0.3
PostC	14	26.6 ± 0.5	2.5 ± 0.2	351 ± 12	135 ± 4	8.5 ± 0.3	−5.9 ± 0.2	1.7 ± 0.1	320 ± 11	67 ± 5†	5.6 ± 0.3	−4.3 ± 0.2
I/R-SNAP	8	27.9 ± 0.3	2.7 ± 0.2	389 ± 12	125 ± 9	8.0 ± 0.3	−5.2 ± 0.3	1.8 ± 0.1	345 ± 11	54 ± 3*	4.7 ± 0.2	−3.9 ± 0.3
I/R-l-NAME	6	27.2 ± 0.5	2.6 ± 0.1	380 ± 20	147 ± 8	9.1 ± 0.4	−6.2 ± 0.2	2.1 ± 0.2	301 ± 19	44 ± 8	4.2 ± 0.5	−3.7 ± 0.4
l-NAME + PostC	8	26.5 ± 0.6	2.9 ± 0.2	374 ± 11	143 ± 4	9.0 ± 0.4	−6.2 ± 0.4	1.8 ± 0.3	309 ± 13	47 ± 6	4.2 ± 0.5	−3.6 ± 0.4
I/R-ODQ	5	27.3 ± 0.3	2.7 ± 0.3	386 ± 26	129 ± 7	8.1 ± 0.6	−5.5 ± 0.4	1.7 ± 0.2	344 ± 13	30 ± 6	4.0 ± 0.5	−3.3 ± 0.2
ODQ + PostC	7	28.3 ± 0.5	2.5 ± 0.2	379 ± 20	130 ± 7	9.0 ± 0.6	−5.7 ± 0.4	2.0 ± 0.2	334 ± 10	57 ± 4‡	4.7 ± 0.3	−3.9 ± 0.3
I/R-KT5823	4	27.6 ± 0.3	2.5 ± 0.1	416 ± 24	124 ± 6	8.4 ± 0.6	−5.6 ± 0.3	1.9 ± 0.1	314 ± 11	39 ± 5	3.9 ± 0.3	−3.1 ± 0.4
KT5823 + PostC	5	27.6 ± 0.6	2.4 ± 0.1	408 ± 21	127 ± 5	8.5 ± 0.4	−6.3 ± 0.4	1.9 ± 0.1	344 ± 19	65 ± 3§	5.5 ± 0.3	−4.0 ± 0.2

Values are means ± SE; n = no. of hearts. BW, body weight; FR, flow rate; HR, heart rate; LVDP, left ventricular developed pressure; ±dP/dt, rates of pressure rise and fall, respectively; I/R, ischemia-reperfusion; SNAP, S-nitroso-N-acetyl-d,l-penicillamine. l-NAME, N^G-nitro-l-arginine methyl ester; ODQ, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one; PostC, postconditioning;.

^*P < 0.05,

^†P < 0.01 vs. I/R-control;

^‡P < 0.05 vs. I/R-ODQ;

^§P < 0.05 vs. I/R- KT5823.