Table 2.

Proteins identified by 2D CyDye-maleimide DIGE with increased SNO level in PostC hearts

					SNO Level (Arbitrary Ratio)
Spots	Protein Name	Accession No.	MW, kDa	Protein pI	PostC vs. I/R	PostC vs. PostC + l-NAME
1	2-Oxoglutarate dehydrogenase	Q60597	116.4	6.83	1.48 ± 0.15	1.40 ± 0.16
2	Aconitate hydratase, mitochondrial	Q99KI0	85.4	8.08	1.50 ± 0.19	1.38 ± 0.14
3	Mitochondrial F1-ATPase subunit α	Q03265	59.7	9.22	1.47 ± 0.18	1.50 ± 0.13
4	Creatine kinase S-type	Q6P8J7	47.8	8.64	1.45 ± 0.18	1.53 ± 0.14
5	Creatine kinase M-type	P07310	43.0	6.58	1.51 ± 0.28	1.41 ± 0.11
6	α-Cardiac muscle actin	P68033	42.3	5.23	1.45 ± 0.17	1.44 ± 0.16
7	Malate dehydrogenase, cytoplasmic	P14152	36.5	6.16	1.46 ± 0.15	1.49 ± 0.19
8	Glyceraldehyde-3-phosphate dehydrogenase	P16858	35.8	8.25	1.28 ± 0.04	1.35 ± 0.09
9	Malate dehydrogenase, mitochondrial	P08249	35.6	8.68	1.57 ± 0.14	1.45 ± 0.08
10	Electron transfer flavoprotein α	Q99LC5	35.0	8.62	1.54 ± 0.12	1.55 ± 0.14
11	Electron transfer flavoprotein β	Q9DCW4	27.8	8.24	1.46 ± 0.12	1.49 ± 0.13
12	Myosin light chain 1	P09542	22.5	5.03	1.43 ± 0.13	1.50 ± 0.13
13	Myoglobin	P04247	17.1	7.07	1.30 ± 0.05	1.31 ± 0.07

Values are means ± SE. Protein identifications were accepted based on two or more unique peptides with a false discovery rate (FDR) of 99% or higher and a correct molecular mass identification. SNO protein spots showed a change of at least 25% or higher in PostC hearts compared with I/R-control or PostC + l-NAME hearts (P < 0.05, n = 3 in each group).