Table 3.

S-nitrosylation sites identified via SNO-RAC proteomic analysis in PostC hearts

Protein Name	ID	Peptide Sequence	SNO-Cys
Extracellular matrix and cell membrane
Galectin-1	P16045	ACGLVASNLNLKPGECLK	3, 17
Long-chain fatty acid transport protein 1	Q60714	VGSCGFNSR	406
PDZ and LIM domain protein 5	Q8CI51	ACTGSLNMTLQR	73
Protein-glutamine gamma-glutamyltransferase 2	P21981	YSGCLTESNLIK	553
Cytoplasm and cytoskeleton
6-Phosphofructokinase, muscle type	P47857	LPLMECVQVTK	351
		IFANTPDSGCVLGMR	709
Alpha-enolase*	P17182	VNQIGSVTESLQACK	357
Annexin A6	P14824	GTVCAANDFNPDADAK	358
Cytoplasmic dynein 1 heavy chain 1	Q9JHU4	VQYPQSQACK	631
		LQGATCSNNK	4568
Destrin	Q9R0P5	ASGVQVADEVCR	12
Dihydropyrimidinase-related protein 2	O08553	GLYDGPVCEVSVTPK	504
E3 ubiquitin-protein ligase UBR4	A2AN08	AVQCLNTSSK	2552
Elongation factor 2	P58252	ETVSEESNVLCLSK	591
Filamin-B	Q80X90	VAVTEGCQPSR	1326
Fructose-bisphosphate aldolase A*	P05064	ALANSLACQGK	339
Glutaredoxin-1	Q9QUH0	AQEFVNCK	8
Glyceraldehyde-3-phosphate dehydrogenase*	P16858	IVSNASCTTNCLAPLAK	150, 154
		VPTPNVSVVDLTCR	245
Heat shock protein HSP 90-β	P11499	FENLCK	564
l-Lactate dehydrogenase A chain	P06151	VIGSGCNLDSAR	163
Myosin-binding protein C, cardiac-type	O70468	ATNLQGEAQCECR	1260, 1262
Myosin light chain kinase, smooth muscle	Q6PDN3	VAGTQPITCK	1291
Obscurin	A2AAJ9	QADTGTVCATSPK/	3060
Rab GDP dissociation inhibitor β	Q61598	TDDYLDQPcCETINR	203
Selenium-binding protein 1	P17563	GGSVQVLEDQELTCQPEPLVVK	371
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B α isoform	Q6P1F6	AGAGGGNDIQWCFSQVK	13
Titin*	A2ASS6	VSECYVAR	25303
		VLDSPGPCGK	30933
Trifunctional purine biosynthetic protein adenosine-3	Q64737	QVLVAPGNAGTACAGK	41
Triosephosphate isomerase	P17751	IAVAAQNCYK	117
		IIYGGSVTGATCK	268
Tripartite motif-containing protein 72	Q1XH17	MQLQEACMR	144
		FCLVTSR	242
Tubulin β-4A chain	Q9D6F9	NMMAACDPR	303
		TAVCDIPPR	354
Ubiquitin-like modifier-activating enzyme 1	Q02053	DNPGVVTCLDEAR	234
Endo/sarcoplasmic reticulum, nucleus, ribosome
Bifunctional glutamate/proline-tRNA ligase	Q8CGC7	VACQGEVVR	910
Cellular nucleic acid-binding protein	P53996	TSEVNCYR	159
eIF4E-binding protein 1	Q60876	SAGSSCSQTPSR	7
Nascent polypeptide-associated complex subunit α	P70670	GTVVCLADSSLDTSVSASK	755
Poly(rC)-binding protein 1	P60335	INISEGNCPER	54
40S ribosomal protein S3	P62908	GCEVVVSGK	134
40S ribosomal protein S28	P62858	TGSQGQCTQVR	27
60S ribosomal protein L10	Q6ZWV3	MLSCAGADR	105
60S ribosomal protein L27a	P14115	NQSFCPTVNLDK	70
Sarco/endoplasmic reticulum calcium ATPase 2	O55143	SLPSVETLGCTSVICSDK	344, 349
		TGTLTTNQMSVCR	364
Mitochondria
2-Oxoglutarate dehydrogenase	Q60597	ICEEAFTR	566
3-Ketoacyl-CoA thiolase	Q8BWT1	YAVGSACIGGGQGIALIIQNTA	382
Acetyl-CoA acetyltransferase	Q8QZT1	QATLGAGLPISTPCTTVNK	116
ATP synthase subunit ϵ	P56382	FSQICAK	19
Aconitate hydratase	Q99KI0	VGLIGSCTNSSYEDMGR	385
Aspartate aminotransferase	P05202	VGAFTVVCK	295
Carnitine O-acetyltransferase	P47934	IYGQACATYESASLR	449
Carnitine O-palmitoyltransferase 1	Q924X2	SCTNESAAFVQAMMK	608
Citrate synthase	Q9CZU6	LPCVAAK	211
Creatine kinase S-type	Q6P8J7	GLSLPPACSR	180
Cytochrome b-c1 complex subunit 1*	Q9CZ13	LCTSATESEVTR	380
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex	Q8BMF4	DVPLGAPLCIIVEK	290
Dynamin-1-like protein	Q8K1M6	FATEYCNTIEGTAK	351
Electron transfer flavoprotein-ubiquinone oxidoreductase	Q921G7	ASCDAQTYGIGLK	265
Enoyl-CoA hydratase	Q8BH95	TFQDCYSSK	111
		LVEEAIQCAEK	225
Glutathione S-transferase κ 1	Q9DCM2	LIENTDAACK	176
Iron-sulfur cluster assembly 2 homolog	Q9DCB8	LTDSCVQR	56
Isocitrate dehydrogenase [NADP]*	P54071	SSGGFVWACK	308
		VCVQTVESGAMTK	402
Isocitrate dehydrogenase [NAD] subunit α	Q9D6R2	IEAACFATIK	331
		cSDFTEEICR	359
Lactation elevated protein 1	Q3V384	VVQCLQK	100
Leucine-rich PPR motif-containing protein	Q6PB66	LIAAYCNVGDIEGASK	207
Malate dehydrogenase*	P08249	GYLGPEQLPDCLK	89
		EGVVECSFVQSK	275
Methylmalonate-semialdehyde dehydrogenase	Q9EQ20	VCNLIDSGTK	368
Mitochondrial tRNA-specific 2-thiouridylase 1	Q9DAT5	TPNPDINCNK	101
Mitofusin-1	Q811U4	LCQQVDVTQK	681
NADH-ubiquinone oxidoreductase 75 kDa subunit	Q91VD9	LSVAGNCR	75
		AVTEGAQAVEEPSIC	727
NADH-ubiquinone oxidoreductase chain 3	P03899	ANPYECGFDPTSSAR	39
Propionyl-CoA carboxylase α chain	Q91ZA3	MADEAVCVGPAPTSK	107
Short/branched chain specific acyl-CoA dehydrogenase	Q9DBL1	ASSTCQLTFENVK	261
Succinate dehydrogenase cytochrome b560 subunit	Q9CZB0	SLCLGPTLIYSAK	107
Succinate dehydrogenase [ubiquinone] flavoprotein subunit	Q8K2B3	TLNEADCATVPPAIR	654
Succinate-semialdehyde dehydrogenase	Q8BWF0	NAGQTCVCSNR	328, 330
Succinyl-CoA ligase subunit α	Q9WUM5	LIGPNCPGVINPGECK	172, 181
Succinyl-CoA ligase subunit β	Q9Z2I9	IcNQVLVCER	158
		ILACDDLDEAAK	430
Voltage-dependent anion-selective channel-1	Q60932	YQVDPDACFSAK	245
Voltage-dependent anion-selective channel-2*	Q60930	SCSGVEFSTSGSSNTDTGK	48
Voltage-dependent anion-selective channel-3	Q60931	cNTPTYCDLGK	8

S-nitrosylation (SNO) cysteine residues (SNO-Cys) are labeled in upper case and underlined (C); cysteine residues blocked by N-ethylmaleimide (NEM) are labeled in lower case (c). Protein identifications were accepted based on two or more unique peptides with a FDR of 99% or higher. *SNO-modified proteins and peptides were also identified from 2 of 3 SNO-RAC/LC-MS/MS proteomic analyses. SNO-Cys indicates the amino acid in the protein that is SNO.