Fig. 7.

Grhl2 expression pattern in double-mutant HPS1 and HPS2 (HPS1/2) lung tissue is altered. Double staining of Grhl2 and its target (Cdh1) in normal adult lung tissue (A–C) and HPS1/2 lung tissue (D–F). C′ and F′ are insets. White arrow in C′ and F′ indicate epithelial cells. Insets do not show DAPI for clarity. Scale bar for A–F is 100 μm and for C′ and F′ it is 20 μm. G: gene expression analysis of Grhl2 and Cdh1 was performed in triplicates from the same paraffin sections after recovery of RNA as described in materials and methods. Gapdh was used as endogenous controls.