Fig. 3.

Analysis of DUOX1-dependent protein S-glutathionylation using BioGEE. H292-CTL or H292-shDUOX1 cells (A) or MTE cell from either wild-type or DUOX1 knockout mice (B) were preloaded with BioGEE (250 µM; 1 h), stimulated with ATP (100 µM; 15 min), and cell lysates were mixed with non-reducing sample buffer for analysis by SDS-PAGE, and biotin-labeled proteins were detected by blotting with streptavidin-HRP and enhanced chemiluminescence. Representative blots of 2–3 independent experiments are shown.