Fig. 4.

DUOX1 activation promotes S-glutathionylatoin of proteins involved in cell migration. H292-CTL and H292-shDUOX1 cells (A) or MTE cells from wild-type (WT) or DUOX1-deficient (DUOX1-KO) mice (B) were seeded on 8 µm polycarbonate filters coated with fibronectin and cell migration by haptotaxis was evaluated in the absence or presence of ATP (100 µM) over 24 h, and quantified and expressed relative to unstimulated H292 cells. Mean S.E. (n=4). : p<0.05 compared to unstimulated control; ^#: p<0.05 compared to corresponding treatment of H292-CTL or WT MTE cells. BioGEE-preloaded H292-CTL or H292-shDUOX1 cells (C) or MTE cells from WT or DUOX1-KO mice (D) were stimulated with ATP and biotinylated proteins were collected using neutravidin beads, and analyzed by SDS-PAGE and Western blotting with antibodies against β-actin, Prx1, MKP-1, or Src. Corresponding whole cell lysates were evaluated as input controls. Representative blots of 2 independent experiments are shown.