Fig. 1.

CB3 inactivates JNK and p38 but not ERK1/2 in the brains of ZDF rats. ZDF rats were supplemented with either CB3 or Rosi for 28 days (as described in Table 1). Brain samples of each animal from each group were homogenized and proteins were separated by SDS-PAGE (Section 2). The blots were incubated with antibodies against (A) p38^MAPK phospho-p38^MAPK and β-catenin (B) JNK and phospho-JNK or (C) ERK1/2 and phospho-ERK 1/2. Each band represents a single animal of each group. The values were quantified shown as the averages (±SEM) of all the bands presented in the blots (right). The values were normalized to the phosphorylation state of ZDF rats treated with saline only (Zucker). Student's t test (two populations) was performed for ZDF rats treated with saline only (Zucker). ^*P value<0.05; ^**P value<0.01; and ^***P value<0.005, (n=4–8).