Fig. 3.

CB3 reduces TXNIP/TBP-2 levels in the brain of ZDF rats and in SH-SY5Y cells. ZDF rats were supplemented with either CB3 or Rosi for 28 days as indicated in Fig. 1. Brain samples were lysed and proteins were separated on SDS-PAGE (A) left, TXNIP/TBP-2, levels were determined using TXNIP/TBP-2 antibodies using anti GAPDH antibodies as a reference. Right, all values of each group were collected and normalized to GAPDH. (B) SH-SY5Y cells were exposed to increasing concentrations of CB3, as indicated. The level of TXNIP/TBP-2 was determined using anti TXNIP antibodies (left), and the data was quantified using GAPDH as a reference (right). The results represent the averages (±SEM) of all the bands presented in the blots. All values were normalized to the TXNIP/TBP-2 levels of ZDF rats treated with saline only (Zucker) or to the levels of control cells. Student's t test (two populations) was performed for ZDF rats treated with saline only (Zucker) or to control cells. ^*P value<0.05; ^**P value<0.01; and ^⁎⁎⁎P value<0.005, (n=4–8).