Fig. 6.

CB3 and CB4 inhibit caspase 3 and PARP dissociation in SH-SY5Y cells. (A) SH-SY5Y cells were treated for 24 h with or without CB3 at the concentrations as indicated. Equal proteins of whole-cell lysates were separated by SDS-PAGE. Caspase 3 cleavage was detected using antibodies against cleaved caspase-3. (B) Increasing concentrations of CB3 or CB4 were tested for preventing AuF-induced PARP dissociation. PARP dissociation was detected using antibodies against PARP. The values were quantified as shown (right) are averages (±SEM) of three independent experiments. Student's t test (two populations) was performed for either control or AuF treated cells in B. ^*P value<0.05; and ^***P value<0.005.