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. 2014 Mar 11;5:82. doi: 10.3389/fpls.2014.00082

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Copyright © 2014 Murcha, Kubiszewski-Jakubiak, Wang and Whelan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interactions between AtTim21-like 1, AtTim21-like 2 and components of the respiratory chain and import apparatus. Yeast 2-Hybrid interactions indicate a positive interaction between AtTim21-like 1 and 2 and AtTim21 and various components of the mitochondrial import apparatus (AtTim17-2, AtTim23-2, AtTim50, AtTim44-2, AtTim22), Complex I (AtB14.7), Complex III (AtCyc1-1, AtRISP, AtMPPα). AtTim21 was also able to interact with TOM subunits, AtTom9, and AtTom5. Positive diploids are indicated by growth on Double Drop out media (DDO) and protein-protein interactions are indicated by growth of diploid strains on Quadruple Drop Out media (QDO). No interactions of pGBK AtTim21-like 1 and 2 and pGBK AtTim21 were observed against the pGAD empty vector. SV40 and p53 was used as a positive control.