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. 2014 Mar 11;5:26. doi: 10.3389/fneur.2014.00026

Figure 4.

Figure 4

Comparison of western blot profiles between JNPL3 and non-tg mice. (A) Representative blots of S3 fraction stained by tau antibodies (E1, Tau5, MS06, Tau1, pS199, pT231, and pS396). Molecular weights were determined by reference standard markers. Two major bands were migrated to 50 and 55 kDa. Except Tau1 antibody, the 55 kDa band was more intensely stained than 50 kDa band. (B) Representative blots of LP1 fraction stained by tau antibodies (E1, Tau5, MS06, Tau1, pS199, pT231, and pS396). In JNPL3 mouse, two major bands appeared with slower migration (53 kDa) and faster migration (51 kDa) in E1, Tau5, pS199, and pS396 blots. Two bands appeared on 49 and 51 kDa in MS06 blot. Three bands (49, 51, and 53 kDa) appeared in Tau1 blot. Only 51 kDa band appeared in pT231 blot. In non-tg mouse, 49 and 51 kDa bands appeared in Tau5, MS06, and Tau1 blots. Three bands (49, 51, and 53 kDa) appeared in pS199 blot. Only 51 kDa band appeared in pT231 and pS396 blots.