Figure 1.
Effect of zinc and MeJA treatments on PDF1 transcript accumulation in shoots and roots within A. thaliana and A. halleri. The ratios of REL as normalized to actin and to control conditions are presented for each PDF1 in shoots (A) and roots (B). For each organ (A,B), the AtPDF1 and AhPDF1 REL ratios are organized according to orthologous loci (separated by plain lines) and the gene-to-gene orthologous relationship (indicated by dashed lines). Shoots and roots were collected separately from individual A. halleri (left panel) and A. thaliana (right panel) plants (n = 6) that had been transferred to and maintained for 5 days on control media or to media containing either 5 μM MeJA (light green), or 50 μM MeJA (dark green) or 100 μM ZnSO4 (gray). In A. halleri, AhPDF1.2a, AhPDF1.2c, AhPDF1.7 and AhPDF1.8b transcripts were not detected in the shoots or roots. AhPDF1.2b transcripts were not detected in roots. In A. thaliana, AtPDF1.2b and AtPDF1.2c transcripts were not detected in roots. PDF1s shown in red had a significant transcript REL ratio in response to MeJA (see Supplementary Table 4 for bootstrapped 95% confidence interval). Stars indicate the statistical significance of the ratio (P < 0.05 according to bootstrapped 95% confidence intervals). ND, not detected.