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. 2014 Jan 21;352(1):54–61. doi: 10.1111/1574-6968.12368

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© 2013 The Authors. FEMS Microbiology Letters published by John Wiley & Sons Ltd on behalf of Federation of European Microbiological Societies.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Overexpression of PstS and DING in constructed plasmids enhances the production of PstS/DING appendages in PA14. (a–c) TEM images of (a) PA14/pBBR1MCS-5-DING, (b) PA14/pME6032-pstS, (c) PA14/pBBR1MCS-5-DING/pME6032- pstS with overproduction of PstS and DING. The images are taken at magnification of 25 000 × 1.4. (d and e) Immunoassay analysis of sheared fractions using anti-PstS (d) and anti-DING (e) antibodies.