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. 2014 Jan 21;352(1):54–61. doi: 10.1111/1574-6968.12368

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© 2013 The Authors. FEMS Microbiology Letters published by John Wiley & Sons Ltd on behalf of Federation of European Microbiological Societies.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Comparative analysis of PstS and DING expression in PA14 and MDR25. (a) QRT-PCR analysis demonstrating > 20 higher expression of DING in MDR25 compared with PA14. The expression of pstS and DING was normalized to PA4748. (b) C_t levels of PA4748, pstS, and DING determined by quantitative PCR using 1 ng DNA as a template demonstrating no differences in gene copy numbers. (c) Immunoblot analysis of PstS and DING production by PA14 and MDR25 grown in citrate defined media varying in phosphate concentrations demonstrating that MDR25 has higher threshold sensitivity to phosphate level. (d) Quantitative evaluation of immunoblot analysis. n = 3/group, *P < 0.01.