Figure 4. T cells from responder tumors exhibit increased ability to lyse tumor cells.

Tumors were injected and irradiated as described in figure 1 and processed into a single cell suspension. T cells were isolated as described in the materials and methods and examined for the ability to lyse ⁵¹Cr labeled Colon38 tumor cells in a standard 6-hour assay. Specific lysis was determined from various effector to target ratios. * represents significance (p < 0.05) compared to non-responders as determined by ANOVA and Bonferroni post-hoc test. # represents significance (p < 0.05) compared to T cells from unirradiated tumors as determined by ANOVA and Bonferroni post-hoc test. n=4 for unirradiated and 3 for both responders and non-responders.