Figure 7.

Empirical phase diagrams(EPDs) of the peptide drug pramlintide at low and high concentration,⁷ and concentration dependence at pH 4. Low concentrations (0.088 mg/ml) are represented in A-C. The experimental techniques used to construct A-C were as follows: (A) second derivative UV absorbance peak shift and OD₃₅₀, (B) same as (A), adding fluorescence intensity and peak shift, (C) same as (B), adding the CD change at 204 nm. The peptide at high concentration (8.8 mg/mL) is represented in (D), using the same experimental techniques as (B). An EPD at pH 4 as a function of peptide concentration is shown in (E), using the same experimental techniques as (B).